Establishment and Characterization of a New Human Leukemia Cell Line Derived From
نویسندگان
چکیده
A new human leukemia cell line, designated as ME-1, was established from the peripheral blood leukemia cells of a patient with acute myelomonocytic leukemia with eosinophilia (M,E,). This cell line has the characteristic chromosome abnormality of M,E,, inv(l6) (p13q22). When cultured in RPMI 1640 medium containing 10% fetal calf serum, ME-1 cells were monoblastoid, but with the addition of cytokines such as interleukin-3 (IL-3). granulocyte-macrophage colonystimulating factor (GM-CSF), IL-4, or medium conditioned by phytohemagglutinin-stimulated human peripheral leukocytes (PHA-LCM), the cells exhibited differentiation to macrophage-like cells. PHA-LCM also promoted eosinophiliclineage differentiation of this cell line, although IL-5 did not do so. To elucidate the mechanism of proliferation and
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